How To Make A 10 Fold Serial Dilution
More How To Make A 10 Fold Serial Dilution videos.
5 Fold Dilution
2-fold dilution is a bit confusing, a better way of describing dilution is ratio. For example, 2 fold dilution equals to 1:2 dilution. Since you mentioned serial dilution, you do this by first mixing an equal volume of bacteria and water (whatever used for dilution), and then mix an equal volume of the first mixture and water. For 10 fold serial dilution, you do the above 10 times. If you don't mind the intermediate steps (for example you don't need serial concentrations), you can jump to the final dilution by omitting the middle ones, and do this by calculation.
For example, if you want to have 75 ul 10-fold diluted bacteria, the volume of original bacteria needed is x, x = 75 ul /10 fold = 7.5 ul you take 7.5 ul bacteria and add 62.5 ul water to make 75 ul final solution.
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