Rainin Pipette Calibration Tool
A2LA 17025 Accredited Pipette Calibration Services of all major brands. Nationwide On-site or mail-in service, with 24 hour turnaround. Pipetman, Rainin Classic Calibration Tool. 5 prong spanner wrench designed for the Gilson Pipetman, Gilson Pipetman G, Gilson Pipetman Neo and the Rainin Classic.
Various pipettes and related items: 1) Adjustable pipettes, from left: 20–200 μl, 2–20 μl, 100–1000 μl; 2) Graded transfer pipette and electric pipette filler; 3) 25 mL, 10 mL, 5 mL, and 2 mL transfer pipettes; 4) Disposable tips for adjustable pipettes; 5) 12-channel adjustable pipette for microplates; 6) Low-retention 0.5–10 μl adjustable pipette; 7) Squeezable transfer pipettes; 8) Digital adjustable pipette; 9) Light-guided pipetting system A pipette (sometimes spelled pipet) is a laboratory tool commonly used in, and to transport a measured volume of liquid, often as a. Pipettes come in several designs for various purposes with differing levels of, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid. Measurement accuracy varies greatly depending on the style. Contents.
History The first simple pipettes were made in glass, such as. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required. The first micropipette was patented in 1957 by Dr (, Germany).
The founder of the company, Dr. Heinrich Netheler, inherited the rights and started the commercial production of micropipettes in 1961.
The adjustable micropipette is a Wisconsin invention developed through interactions among several people, primarily inventor Warren Gilson and Henry Lardy, a professor of biochemistry at the. Nomenclature Although specific descriptive names exist for each type of pipette, in practice any type of pipette will merely be referred to as a 'pipette' and the desired device will be obvious from context. Sometimes, pipettes that dispense between 1 and 1000 are distinguished as micropipettes, while macropipettes dispense greater volumes.
Common pipettes Air displacement micropipettes. Main article: Air displacement micropipettes are a type of adjustable micropipette that deliver a measured volume of liquid; depending on size, it could be between about 0.1 to 1000 µl (1 ml). These pipettes require disposable tips that come in contact with the fluid. The four standard sizes of micropipettes correspond to four different disposable tip colors: Pipette type Volumes (μL) Tip color P10 0.5 – 10 white P20 2–20 yellow P200 20–200 yellow P1000 200–1000 blue P5000 1000–5000 white These pipettes operate by -driven displacement. A vacuum is generated by the vertical travel of a metal or ceramic piston within an airtight sleeve. As the piston moves upward, driven by the depression of the plunger, a vacuum is created in the space left vacant by the piston.
The liquid around the tip moves into this vacuum (along with the air in the tip) and can then be transported and released as necessary. These pipettes are capable of being very precise and accurate. However, since they rely on air displacement, they are subject to inaccuracies caused by the changing environment, particularly temperature and user technique. For these reasons this equipment must be carefully maintained and calibrated, and users must be trained to exercise correct and consistent technique.
The micropipette was invented and patented in 1960 by Dr., Germany. Afterwards, the co-founder of the biotechnology company, Dr. Heinrich Netheler, inherited the rights and initiated the global and general use of micropipettes in labs. In 1972, the adjustable micropipette was invented at the University of Wisconsin-Madison by several people, primarily Warren Gilson and Henry Lardy.
Micropipettes brands include Gilson, ErgoOne, Eppendorf, Hamilton, Rainin, Drummond, BrandTech, Oxford, Hirschmann, Biohit, Labnet, Nichiryo, Socorex, Corning, VistaLab, Thermo, Jencons, Vertex, Handypett, Microlit and Pricisexx. Types of air displacement pipettes include:. adjustable or fixed. volume handled. Single-channel, multi-channel or repeater. conical tips or cylindrical tips. standard or locking.
manual or electronic. manufacturer Irrespective of brand or expense of pipette, every micropipette manufacturer recommends checking the calibration at least every six months, if used regularly. Companies in the drug or food industries are required to calibrate their pipettes quarterly (every three months). Schools which are conducting classes can have this process annually. Those studying forensics and research where a great deal of testing is commonplace will perform monthly calibrations. Positive displacement pipette.
Main article: are a type of macropipette consisting of a long tube with a series of graduations, as on a or, to indicate different calibrated volumes. They also require a source of vacuum; in the early days of chemistry and biology, the mouth was used. The safety regulations included the statement: 'Never pipette by mouth KCN, NH3, strong acids, bases and mercury salts'. Some pipettes were manufactured with two bubbles between the mouth piece and the solution level line, to protect the chemist from accidental swallowing of the solution. Pasteur pipettes with rubber bulbs attached. Are plastic or glass pipettes used to transfer small amounts of liquids, but are not graduated or calibrated for any particular volume. The bulb is separate from the pipette body.
Pasteur pipettes are also called pipettes, droppers, eye droppers and chemical droppers. The proper handling of the pipettes is by holding the rubber bulb between the thumb and index finger and controlling the glass by holding it between the ring finger and the pinky. The middle finger is not used.
Transfer pipettes. This section needs additional citations for. Unsourced material may be challenged and removed. (July 2012) Pipetting syringes are hand-held devices that combine the functions of volumetric (bulb) pipettes, graduated pipettes, and. They are calibrated to volumetric A grade standards. A glass or plastic pipette tube is used with a thumb-operated piston and seal which slides within the pipette in a positive displacement operation. Such a device can be used on a wide variety of fluids (aqueous, viscous, and volatile fluids; hydrocarbons; essential oils; and mixtures) in volumes between 0.5 mL and 25 mL.
This arrangement provides improvements in precision, handling safety, reliability, economy, and versatility. No disposable tips or pipetting aids are needed with the pipetting syringe. Van Slyke pipette A graduated pipette commonly used in with serologic pipettes for volumetric analysis. Ostwald–Folin pipette A special pipette used in measuring viscous fluid such as whole blood. Common in medical technology laboratory setups together with other pipettes.
Invented by, a Baltic German Chemist and later refined by, an American chemist. Glass micropipette. A pipette, housed in a manifold holder. The colored solutions highlight the solutions loaded into the wells of the pipette. Pneumatic actuation is used to keep all tubing free of contamination. A recent introduction into the micropipette field integrates the versatility of into a freely positionable pipette platform. At the tip of the device a localized flow zone is created, allowing for constant control of the nanoliter environment, directly in front of the pipette.
The pipettes are made from (PDMS) which is formed using reactive injection molding. Interfacing of these pipettes using pneumatics enables multiple solutions to be loaded and switched on demand, with solution exchanged times of 100ms. Invented by Alar Ainla, currently situated in the Biophysical Technology Lab at in Sweden. The technology became commercial in 2013, under the name Multifunctional pipette, with the company. Extremely low volume pipettes A pipette has been developed. The pipette is made of a carbon shell, within which is an alloy of gold-germanium.
The pipette was used to learn about how takes place. An example of pipettes manipulated by an anthropomorphic robot are capable of manipulating the pipettes as humans would do. Calibration Pipette recalibration is an important consideration in laboratories using these devices. It is the act of determining the accuracy of a measuring device by comparison with traceable reference standards. Pipette calibration is essential to ensure that the instrument is working according to expectations and as per the defined regimes or work protocols.
Pipette Calibration Sop
Pipette calibration is considered to be a complex affair because it includes many elements of calibration procedure and several calibration protocol options as well as makes and models of pipettes to consider. Posture and Injuries Proper pipetting posture is the most important element in establishing good ergonomic work practices. During repetitive tasks such as pipetting, maintaining body positions that provide a maximum of strength with the least amount of muscular stress is important to minimize the risk of injury. A number of common pipetting techniques have been identified as potentially hazardous due to biomechanical stress factors.
Recommendations for corrective pipetting actions, made by various US governmental agencies and ergonomics experts, are presented below. Winged elbow pipetting Technique: elevated, “winged elbow”.
The average human arm weighs approximately 6% of the total body weight. Holding a pipette with the elbow extended (winged elbow) in a static position places the weight of the arm onto the neck and shoulder muscles and reduces blood flow, thereby causing stress and fatigue. Muscle strength is also substantially reduced as arm flexion is increased.
Corrective action: Position elbows as close to the body as possible, with arms and wrists extended in straight, neutral positions (handshake posture). Keep work items within easy reach to limit extension and elevation of arm.
Rainin Pipette Calibration Form
Arm/hand elevation should not exceed 12” from the worksurface. Over rotated arm pipetting Technique: Over-rotated forearm and wrist.
Rotation of the forearm in a supinated position (palm up) and/or wrist flexion increases the fluid pressure in the carpal tunnel. This increased pressure can result in compression of soft tissues like nerves, tendons and blood vessels, causing numbness in the thumb and fingers. Corrective action: Forearm rotation angle near 45° pronation (palm down) should be maintained to minimize carpal tunnel pressure during repetitive activity. Clenched fist pipetting Technique: Tight grip (clenched fist).
Hand fatigue results from continuous contact between a hard object and sensitive tissues. This occurs when a firm grip is needed to hold a pipette, such as when jamming on a tip, and results in diminished hand strength. Corrective action: Use pipettes with hooks or other attributes that allow a relaxed grip and/or alleviate need to constantly grip the pipette. This will reduce tension in the arm, wrist and hand. Thumb plunger pipetting Technique: Concentrated area of force (contact stress between a hard object and sensitive tissues). Some devices have plungers and buttons with limited surface areas, requiring a great deal of force to be expended by the thumb or other finger in a concentrated area.
Corrective action: Use pipettes with large contoured or rounded plungers and buttons. This will disperse the pressure used to operate the pipette across the entire surface of the thumb or finger, reducing contact pressure to acceptable levels. Incorrect posture can have a strong impact on available strength arm strength pipetting Technique: elevated arm. Muscle strength is substantially reduced when arm flexion is increased. Corrective action: Keep work items within easy reach to limit extension and elevation of arm. Arm/hand elevation should also not exceed 12” from the worksurface. Elbow strength pipetting Technique: Elbow flexion or abduction.
Arm strength diminishes as elbow posture is deviated from a 90° position. Corrective action: Keep forearm and hand elevation within 12” of the worksurface, which will allow the elbow to remain near a 90° position. Unlike traditional axial pipettes, ergonomic pipetting can affect posture and prevent common pipetting injuries such as carpal tunnel syndrome, tendinitis and other musculoskeletal disorders. To be 'ergonomically correct' significant changes to traditional pipetting postures are essential, like: minimizing forearm and wrist rotations, keeping a low arm and elbow height and relaxing the shoulders and upper arms. References. Retrieved 3 March 2016. Klingenberg, M (2005).
6: 797–800. Zinnen, Tom (June 2004), retrieved November 12, 2011. Shohl, Alfred T. (February 1928). 'A Pipet for Micro-Analyses'.
Journal of the American Chemical Society. 50 (2): 417–417. Retrieved 3 March 2016. Retrieved 3 March 2016. Ainla, Alar; Jansson, Erik T.; Stepanyants, Natalia; Orwar, Owe; Jesorka, Aldo (June 2010). 'A Microfluidic Pipette for Single-Cell Pharmacology'. Analytical Chemistry.
82 (11): 4529–4536. Aimee Cunningham (2007-04-18). Science News., August 2012, retrieved August 29, 2012.
Retrieved 3 March 2016. Retrieved 15 August 2017. Retrieved 3 March 2016. Retrieved 3 March 2016.
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by Oliver Seely.
Regular service greatly improves the consistent performance of pipettes. But does shipping to and from service locations adversely affect the precision of their calibration? This white paper examines the potential risk. It presents findings from two sources: first, a randomized shipping experiment involving multiple packages of pipettes shipped to multiple locations, and second, a case study drawn from a major-account customer that ships thousands of pipettes to Rainin's Massachusetts facility for calibration. Evaluate the risk of pipette damage due to shipping. The designs of most pipette tip racks have not changed for many years, and come from an era when autoclaving was necessary to eliminate any nucleases or microorganisms which may have been present on the tips.
METTLER TOLEDO Rainin is the pioneer of high purity ‘BioClean’ tips, creating a range of super-clean tips which are certified to be free of biological contaminants. Gamma or electron (e-beam) irradiation can be used to sterilize the tips, eliminating the need of autoclaving. Given the reduced need to autoclave and an increased concern about the lasting environmental impact of plastic, the time to develop greener tip rack packaging solutions is now. This white paper compares different rack options available with regard to rack weight, type of plastic and resulting carbon footprint. It shows that Rainin TerraRack is an easy recyclable and low environmental impact tip packaging. We offer a wide selection of pipettes.
Manual pipette. Electronic pipette. Multichannel pipette and single channel pipette. BioClean pipette tips. And expert pipette services that work together as a complete pipetting solution – Pipetting 360° Top quality, innovative design and state-of-the-art production result in superior pipette products with years of reliable operation. In addition, METTLER TOLEDO's unique Good Pipetting Practice™ (GPP™) is a risk-based, comprehensive and systematic approach to maximizing pipetting accuracy and repeatability. Rainin pipette – serving the life sciences for over 50 years.
Rainin manual pipette Rainin offers state-of-the-art single channel pipettes, multichannel pipettes and adjustable spacer manual pipettes and accessories that raise the bar on comfort, performance and control. Rainin's Pipet-Lite™ XLS+ manual pipettes blend cutting-edge engineering and innovation to deliver highly reproducible results with exceptional comfort. New 'stiction-less' elastomeric seal technology and polymer tip ejectors deliver smoothness and control.
The sure-fit handle, light springs and patented LTS™ LiteTouch™ System ensure smooth, comfortable operation and significantly reduce the risk of repetitive strain injuries. Tip shaft options include low-force LTS for improved ergonomics, as well as universal-fit. In addition, all Rainin XLS+ pipettes are equipped with RFID tags for enhanced security, state-of-the-art inventory control and calibration tracking.
Rainin manual pipette – the pipette of choice for world-class results. Rainin electronic pipette Rainin delivers feature-rich single channel pipettes, multichannel pipettes and adjustable spacer electronic pipettes and accessories that are ready for your most demanding laboratory applications. Easily configure the E4 XLS+ to be as simple or elaborate as your work requires. Its large color screen, joystick control and graphic interface make moving between functions and operating the pipette easy and effortless. Not only is every E4 XLS+ function highly customizable, multiple protocols can be stored on board for later use. Pipette settings, protocols and service alarms can be password protected for GLP/GMP compliance. What’s more, GLP data, such as service records, cycles and status data is completely tamper proof.
Your data is everything – trust the E4 XLS+ electronic pipette to deliver!